Journal: Frontiers in Medicine

Article Title: Efficacy evaluation and predictive value of IL-20 and Apelin-13 after cataract surgery by phacoemulsification combined with IOL implantation

doi: 10.3389/fmed.2025.1737194

Figure Lengend Snippet: ROC curve of serum IL-20 (A) , serum Apelin-13 (B) , aqueous humor IL-20 (C) , and aqueous humor Apelin-13 (D) levels for predicting poor efficacy of phacoemulsification combined with IOL implantation surgery in cataract patients.

Article Snippet: The human IL-20 ELISA kit (R&D Systems, USA; Catalog # DY724) from lot # 2365891 (expiry: 03/2025) and the human Apelin-13 ELISA kit (Phoenix Pharmaceuticals, USA; Catalog # EK-057-23) from lot # PHX1022 (expiry: 02/2025) were used.

Techniques:

Journal: Frontiers in Medicine

Article Title: Efficacy evaluation and predictive value of IL-20 and Apelin-13 after cataract surgery by phacoemulsification combined with IOL implantation

doi: 10.3389/fmed.2025.1737194

Figure Lengend Snippet: ROC curve of serum IL-20/Apelin-13 ratio (A) and aqueous humor IL-20/Apelin-13 ratio (B) for predicting poor efficacy of phacoemulsification combined with IOL implantation surgery in cataract patients.

Article Snippet: The human IL-20 ELISA kit (R&D Systems, USA; Catalog # DY724) from lot # 2365891 (expiry: 03/2025) and the human Apelin-13 ELISA kit (Phoenix Pharmaceuticals, USA; Catalog # EK-057-23) from lot # PHX1022 (expiry: 02/2025) were used.

Techniques:

Journal: Scientific Reports

Article Title: Anti-IL-20 monoclonal antibody promotes bone fracture healing through regulating IL-20-mediated osteoblastogenesis

doi: 10.1038/srep24339

Figure Lengend Snippet: ( a ) Levels of IL-20 in serum from mice with bone fracture were analyzed at the indicated time during fracture healing. Values are means ± SD of 6 mice. Data are representative of three independent experiments. * P < 0.05 versus 0 day post-fracture. ( b ) Representative figures of micro-CT analyses of the fracture callus of mice 21 and 42 days post-fracture, followed by no treatment (Control), 3 mg mIgG/kg/3 d treatment, and 3 mg 7E/kg/3 d treatment ( n = 8/group). Images of these individual slices were taken from the central region of each callus. Data are representative of three independent experiments. ( c ) BMD at the fracture callus was assessed using micro-CT scans 21 and 42 days post-fracture ( n = 8/group). Values are means ± SD. Data are representative of three independent experiments. * P < 0.05 versus mIgG controls. ( d ) ALP staining analysis and quantification of the number of osteoblasts in fracture callus 21 days post-fracture ( n = 8/group). Values are means ± SD of 3 frozen sections. Data are representative of three independent experiments. * P < 0.05 versus mIgG controls. ( e ) TRAP staining analysis and quantification of the number of osteoclasts in bone surface 21 days post-fracture ( n = 8/group). Values are means ± SD of 3 frozen sections. Data are representative of three independent experiments. * P < 0.05 versus mIgG controls.

Article Snippet: Serum levels of IL-20 and sclerostin were determined using an IL-20 ELISA kit and a sclerostin ELISA kit (R&D Systems).

Techniques: Micro-CT, Staining

Journal: Scientific Reports

Article Title: Anti-IL-20 monoclonal antibody promotes bone fracture healing through regulating IL-20-mediated osteoblastogenesis

doi: 10.1038/srep24339

Figure Lengend Snippet: ( a ) Levels of IL-20 in serum from IL-20R1 +/+ and IL-20R1 −/− mice with bone fracture were analyzed at the indicated times during fracture healing. Values are means ± SD of 6 mice. Data are representative of three independent experiments. * P < 0.05 versus IL-20R1 +/+ mice 0 day post-fracture. # P < 0.05 versus IL-20R1 −/− mice 0 day post-fracture. ♮ P < 0.05 versus IL-20R1 −/− mice post-fracture. ( b ) Radiographs of fractured right femurs from IL-20R1 +/+ and IL-20R1 −/− mice ( n = 8/group). The radiographic time course is shown at the callus formation for the mice in each group. Data are representative of three independent experiments. ( c ) Radiological assessments on fracture healing by fracture healing stage score of the X-ray images from IL-20R1 +/+ and IL-20R1 −/− mice ( n = 8/group). Data are representative of three independent experiments. ( d ) Representative figures of micro-CT analyses of the fracture callus of IL-20R1 +/+ and IL-20R1 −/− mice 21 and 42 days post-fracture ( n = 8/group). Images of these individual slices were taken from the central region of each callus. Data are representative of three independent experiments. ( e ) BMD at the fracture line was assessed using micro-CT scans 21 and 42 days post-fracture ( n = 8/group). Values are means ± SD. Data are representative of three independent experiments. * P < 0.05 versus IL-20R1 +/+ mice. ( f ) ALP staining analysis and quantification of the number of osteoblasts in the fracture callus 21 days post-fracture ( n = 8/group). Values are means ± SD of 3 frozen sections. Data are representative of three independent experiments. * P < 0.05 versus IL-20R1 +/+ mice. ( g ) TRAP staining analysis and quantification of the number of osteoclasts in bone surface 21 days post-fracture ( n = 8/group). Values are means ± SD of 3 frozen sections. Data are representative of three independent experiments. * P < 0.05 versus IL-20R1 +/+ mice.

Article Snippet: Serum levels of IL-20 and sclerostin were determined using an IL-20 ELISA kit and a sclerostin ELISA kit (R&D Systems).

Techniques: Micro-CT, Staining

Journal: Scientific Reports

Article Title: Anti-IL-20 monoclonal antibody promotes bone fracture healing through regulating IL-20-mediated osteoblastogenesis

doi: 10.1038/srep24339

Figure Lengend Snippet: Level of IL-20 and sclerostin in serum from ( a ) 29 healthy volunteers, ( b ) 10 patients with bone fracture, ( c ) 79 patients with osteopenia, and ( d ) 57 patients with osteoporosis was analyzed. Values ≥0 and ≤0.3: weak positive linear relationship via a shaky linear rule; from >0.3 and ≤0.6: moderate positive linear relationship via a fuzzy-firm linear rule; >0.6 and ≤1.0: strong positive linear relationship via a firm linear rule.

Article Snippet: Serum levels of IL-20 and sclerostin were determined using an IL-20 ELISA kit and a sclerostin ELISA kit (R&D Systems).

Techniques:

Journal: Scientific Reports

Article Title: Anti-IL-20 monoclonal antibody promotes bone fracture healing through regulating IL-20-mediated osteoblastogenesis

doi: 10.1038/srep24339

Figure Lengend Snippet: ( a,b ) hAFSCs were incubated with osteoblast differentiation medium. The osteoblasts were characterized using ALP staining ( a ) 14 days post-incubation, or Alizarin staining ( b ) 28 days post-incubation. ( c ) Cell lysates were collected and measured; ALP activity was measured using an assay kit 14 days post-incubation. Values are means ± SD. * P < 0.05 versus untreated controls (Ctrl). ( d–f ) hAFSCs were cultured under osteogenic conditions for 14 days. mRNA was isolated and the transcripts of OSX, RUNX2, and Atf4 were measured using RTQ-PCR with specific primers. The quantification analysis of mRNA was normalized; GAPDH was the housekeeping gene. * P < 0.05 versus untreated controls (Ctrl). # P < 0.05 versus the 7E-treated group. ( g ) hAFSCs were cultured under osteogenic conditions for 28 days. The expression of sclerostin was analyzed using RTQ-PCR with specific primers. The quantification analysis of mRNA was normalized; GAPDH was the housekeeping gene. * P < 0.05 versus untreated controls. # P < 0.05 versus the IL-20-treated group. All experiments were run three times, with similar results. Data are from a representative experiment.

Article Snippet: Serum levels of IL-20 and sclerostin were determined using an IL-20 ELISA kit and a sclerostin ELISA kit (R&D Systems).

Techniques: Incubation, Staining, Activity Assay, Cell Culture, Isolation, Expressing

Journal: Scientific Reports

Article Title: Anti-IL-20 monoclonal antibody promotes bone fracture healing through regulating IL-20-mediated osteoblastogenesis

doi: 10.1038/srep24339

Figure Lengend Snippet: ( a ) MC3T3-E1 cells were incubated with osteoblast differentiation medium. Their ability to differentiate into osteoblasts was characterized using ALP staining with an assay kit 14 days post-incubation. * P < 0.05 versus untreated controls (Ctrl). ( b ) MC3T3-E1 cells were treated with IL-20 (200 ng/ml), 7E (2 μg/ml), or IL-20+7E for 4 h. Sclerostin and OPG expression were analyzed using RTQ-PCR with specific primers. The quantification analysis of mRNA was normalized; GAPDH was the housekeeping gene. * P < 0.05 versus untreated controls. # P < 0.05 versus the IL-20-treated group. ( c ) MC3T3-E1 cells were pre-incubated with BMP-2 for 2 h, and then treated with IL-20 (200 ng/ml) for another 4 h. OPG expression levels were analyzed using RTQ-PCR with specific primers. The quantification analysis of mRNA was normalized; GAPDH was the housekeeping gene. * P < 0.05 versus untreated controls. # P < 0.05 versus the BMP-2-treated group. All experiments were run three times, with similar results. Data are representative of 3 independent experiments. ( d ) MC3T3-E1 cells were treated with IL-20 (200 ng/ml), 7E (2 μg/ml), or IL-20+7E for 4 h. mRNA was isolated and the transcripts of OSX, Wnt3a, Wnt7a, and Wnt7b were analyzed using RTQ-PCR with specific primers. The quantification analysis of mRNA was normalized; GAPDH was the housekeeping gene. * P < 0.05 versus untreated controls. All experiments were run three times, with similar results. Data are from a representative experiment. ( e ) MC3T3-E1 cells were incubated with mIgG (2 μg/ml), 7E (2 μg/ml), IL-20 (200 ng/ml), IL-20+mIgG or IL-20+7E for 6 h, and cell lysates were collected and analyzed using Western blotting for the indicated protein. Data are representative of three independent experiments. ( f ) HEK293 cells transfected with human OSX promoter construct and incubated with IL-20 (200 ng/ml) for 24 h. Cell lysate were collected and analyzed using a luciferase assay. * P < 0.05 versus untreated controls. All experiments were run three times, with similar results. Data are from a representative experiment.

Article Snippet: Serum levels of IL-20 and sclerostin were determined using an IL-20 ELISA kit and a sclerostin ELISA kit (R&D Systems).

Techniques: Incubation, Staining, Expressing, Isolation, Western Blot, Transfection, Construct, Luciferase

Journal: Scientific Reports

Article Title: Anti-IL-20 monoclonal antibody promotes bone fracture healing through regulating IL-20-mediated osteoblastogenesis

doi: 10.1038/srep24339

Figure Lengend Snippet: Primary mouse preosteoblastic calvaria cells were isolated from 24-h-old IL-20R1 +/+ and IL-20R1 −/− mice and cultured under osteogenic conditions for 21 days. mRNA was isolated, and ( a ) RUNX2 and ( b ) Atf4 transcripts were analyzed using RTQ-PCR with specific primers. All experiments were run three times, with similar results. Data are from a representative experiment. ( c ) Mature osteoblasts derived from IL-20R1 +/+ and IL-20R1 −/− mice were incubated with IL-20 (200 ng/ml) for 6 h. mRNA was isolated and sclerostin transcripts were analyzed using RTQ-PCR with specific primers. * P < 0.05 versus IL-20R1 +/+ untreated cells. All experiments were run three times, with similar results. Data are from a representative experiment.

Article Snippet: Serum levels of IL-20 and sclerostin were determined using an IL-20 ELISA kit and a sclerostin ELISA kit (R&D Systems).

Techniques: Isolation, Cell Culture, Derivative Assay, Incubation

Journal: Scientific Reports

Article Title: Anti-IL-20 monoclonal antibody promotes bone fracture healing through regulating IL-20-mediated osteoblastogenesis

doi: 10.1038/srep24339

Figure Lengend Snippet: ( a ) A schematic representation showing that IL-20 is an inhibitory factor for osteoblastogenesis and promotes osteoclastogenesis. ( b ) Blocking of IL-20 by anti-IL-20 mAb (7E) caused the increased osteoblastogenesis and the inhibited osteoclastogenesis.

Article Snippet: Serum levels of IL-20 and sclerostin were determined using an IL-20 ELISA kit and a sclerostin ELISA kit (R&D Systems).

Techniques: Blocking Assay